Composite

Part:BBa_K3040501:Design

Designed by: Chen, Chi-Wei   Group: iGEM19_NTHU_Taiwan   (2019-09-18)


Lipase-Pseudomonas sp 7323.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 40
    Illegal suffix found in sequence at 2168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal SpeI site found at 2169
    Illegal PstI site found at 2183
    Illegal NotI site found at 46
    Illegal NotI site found at 2176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 40
    Illegal suffix found in sequence at 2169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 40
    Illegal XbaI site found at 55
    Illegal SpeI site found at 2169
    Illegal PstI site found at 2183
    Illegal NgoMIV site found at 436
    Illegal NgoMIV site found at 548
    Illegal NgoMIV site found at 1583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Vigiu


Source

Gyivyig

References